24 veces mayor en jubilados XMH = 2.02, RM = 2.74, chi cuadrada p = 0.03 (IC 95% 1.03 7.30); 1.15 veces mayor en hombres XMH = 1.97, RM = 1.61 chi cuadrada p = 0.04 (IC 95% 1.00-2.60), y 1.21 veces mayor en el grupo de 65 años o más XMH = 2.34, RM = 2.21, chi cuadrada p = 0.01 (IC 95% 1.12-4.35). Conclusiones los resultados de este trabajo son un punto de partida para prevenir, diagnosticar, controlar y dar seguimiento a los casos de TB a nivel delegacional en el IMSS.in English, Spanish Introducción la sepsis es una de las principales causas de morbimortalidad en neonatos. Objetivo identificar los factores de riesgo para sepsis neonatal en una unidad de neonatología durante los meses de marzo a octubre del año 2016. Métodos estudio de casos y controles. Los factores analizados fueron a) factores neonatales como tipo de parto, sexo, peso al nacimiento, edad gestacional, criterios de síndrome de respuesta inflamatoria sistémica, tipo de sepsis (temprana o tardía) y resultado de hemocultivo; b) métodos invasivos como cateterismo central, nutrición parenteral total, cateterismo umbilical y ventilación mecánica y; c) factores maternos como número de controles prenatales, infección durante el embarazo, ruptura prematura de membranas, edad materna y fiebre materna. Se utilizó razón de momios (RM) para determinar asociación. Resultados para el desarrollo de sepsis temprana, los factores de riesgo significativos fueron el desequilibrio termodinámico, la taquicardia y la fiebre materna. Respecto a la sepsis tardía se encontraron asociaciones significativas para el desequilibrio termodinámico, el uso de cateterismo umbilical, la ventilación mecánica y los controles prenatales insuficientes. Conclusión el desequilibrio termodinámico, la taquicardia, la ventilación mecánica, el cateterismo umbilical, la fiebre materna y los controles prenatales insuficientes fueron los probables factores de riesgo significativos asociados a sepsis neonatal.α,β-Thujone is a natural terpenoid found in many medicinal herbs, such as Artemisia absinthium (wormwood), that exhibits antioxidant, anti-diabetic, and anti-tumorigenic effects. α,β-Thujone has numerous functions; it serves as a food ingredient, cosmetic additive, and medicinal remedy. Although the therapeutic properties of α,β-thujone were previously revealed, a comprehensive description of the mechanisms of its anti-cancer potential in choriocarcinoma is yet to be provided. To our knowledge, this study is the first to demonstrate that α,β-thujone attenuates JEG3 and JAR choriocarcinoma cells through a caspase-dependent intrinsic apoptotic pathway. Moreover, α,β-thujone was demonstrated to induce a global mitochondrial defect and ER stress in choriocarcinoma by causing mitochondrial depolarization, calcium overload, and metabolic alterations, thereby leading to energy deprivation, which eventually contributes to the increase in apoptosis of choriocarcinoma cells. Herein, we also revealed the synergistic anti-cancer activity of α,β-thujone via its sensitization effect on paclitaxel in choriocarcinoma cells. Altogether, our findings suggest that α,β-thujone is a novel, natural pharmacological compound that can be used to treat human placental choriocarcinoma.CRISPR-mediated transcriptional activation, also known as CRISPR-on, has proven efficient for activation of individual or multiple endogenous gene expression in cultured cells from several species. However, the potential of CRISPR-on technology in preimplantation mammalian embryos remains to be explored. Here, we report for the first time the successful modulation of endogenous gene expression in bovine embryos by using the CRISPR-on system. As a proof of principle, we targeted the promoter region of either SMARCA4 or TFAP2C genes, transcription factors implicated in trophoblast lineage commitment during embryo development. We demonstrate that CRISPR-on provides temporal control of endogenous gene expression in bovine embryos, by simple cytoplasmic injection of CRISPR RNA components into one cell embryos. dCas9VP160 activator was efficiently delivered and accurately translated into protein, being detected in the nucleus of all microinjected blastomeres. Our approach resulted in the activation of SMARCA expression shortly after microinjection, with a consequent effect on downstream differentiation promoting factors, such as TFAP2C and CDX2. https://www.selleckchem.com/products/nd-630.html Although targeting of TFAP2C did not result in a significant increase in gene expression, in downstream CDX2 expression an induction was detected at day 2 after microinjection. Finally, we demonstrate that CRISPR-on system is suitable for gene expression modulation during the preimplantation period, since no detrimental effect was observed on microinjected embryo development. This study constitutes a first step towards the application of the CRISPR-on system for the study of early embryo cell fate decisions in cattle and other mammal embryos, as well as to design novel strategies that may lead to an improved trophectoderm development.BACKGROUND The novel coronavirus disease 2019 (COVID-19) pandemic is an urgent public health crisis, with epidemiologic models predicting severe consequences, including high death rates, if the virus is permitted to run its course without any intervention or response. Contact tracing using smartphone technology is a powerful tool that may be employed to limit disease transmission during an epidemic or pandemic; yet, contact tracing apps present significant privacy concerns regarding the collection of personal data such as location. OBJECTIVE The aim of this study is to develop an effective contact tracing smartphone app that respects user privacy by not collecting location information or other personal data. METHODS We propose the use of an anonymized graph of interpersonal interactions to conduct a novel form of contact tracing and have developed a proof-of-concept smartphone app that implements this approach. Additionally, we developed a computer simulation model that demonstrates the impact of our proposal on epidemic or pandemic outbreak trajectories across multiple rates of adoption. RESULTS Our proof-of-concept smartphone app allows users to create "checkpoints" for contact tracing, check their risk level based on their past interactions, and anonymously self-report a positive status to their peer network. Our simulation results suggest that higher adoption rates of such an app may result in a better controlled epidemic or pandemic outbreak. CONCLUSIONS Our proposed smartphone-based contact tracing method presents a novel solution that preserves privacy while demonstrating the potential to suppress an epidemic or pandemic outbreak. This app could potentially be applied to the current COVID-19 pandemic as well as other epidemics or pandemics in the future to achieve a middle ground between drastic isolation measures and unmitigated disease spread. ©Tyler M Yasaka, Brandon M Lehrich, Ronald Sahyouni. Originally published in JMIR mHealth and uHealth (http//mhealth.jmir.org), 07.04.2020.